When deciding RT-qPCR experiments, there are two different methods. In this lab chat, Application Specialist Maggie Ma explains the differences between 1-step and 2-step RT-qPCR.

1. To minimize the potential for sample cross-contamination and nucleic acid carryover from one experiment to the next, use aerosol-resistant pipette tips and designated work areas and pipettes for ...

For ethical and safety reasons it is critical to develop easily implemented assays with high sensitivity and specificity for gene doping surveillance. Two nested quantitative real-time PCR (qPCR) ...

SARS-CoV-2 has put unprecedented pressure on medical professionals, public health organizations, and the general public. The accurate and timely diagnosis of SARS-CoV-2 infection is paramount to ...

New England Biolabs® (NEB®) today announced the launch of its Luna Probe One-Step RT-qPCR 4X Mix with UDG, which is optimized for the sensitive detection of target RNA sequences for probe-based ...